![]() ![]() This phase shows an intense metabolic activity of microorganisms due to the production of primary metabolites. ![]() This phase is also called as “ Exponential or Logarithmic phase”. In the lag phase, microorganisms release some metabolites: This phase is characterized by the no increase in cell number. Microorganisms retain in this phase for a short period of time (1hr- several days). This phase is the “ Adaptation or Adjustment phase”, in which the microorganisms adapt themselves to the new environment of the growth medium. There are distinct four phases in the growth curve of bacteria. At last, note down the readings to plot a standard graph between the log number of bacteria and the time interval.After that, incubate the bacteria at different time intervals and determine the bacterial cell count at that given time interval using a spectrophotometer.Then, inoculate it into the fresh culture medium (nutrient broth) that contains all the nutrients for microbial growth.First, take the fresh bacterial sample.To obtain a standard growth curve of bacteria, we need to follow the given protocol: The different phases in the growth cycle of bacteria at the given time interval can be defined as the growth curve of bacteria. Taking log at both the sides in equation 1 To mathematically express the growth of bacteria, there is a relationship that exists between the initial number of cells (in the log phase) and the final number of cells (after the log phase) in the cell culture, which can be expressed as: Here, n indicates the number of generations. a bacterial cell first divides into two, then four, then six, and so on ( x, x 2,x 4,x 6……x n). The growth pattern of bacteria is in a geometric fashion, i.e. Mathematical Expression of Bacterial Growth This transverse septum will divide or separates the cell into two identical daughter cells.Then, the cell wall protrudes into the cytoplasm and form a transverse septum.After the replication, each DNA moves towards the corner of the bacterial cell.Then, the process of replication occurs by which the bacterial cell will form two copies of DNA. ![]()
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